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Hsiang-Yu Wang  - - - 
Top co-authors See all
Bruno Le Pioufle

63 shared publications

CNRS, SATIE, Ecole Normale Supérieure Paris Saclay, Université Paris-Saclay, 61 av du Pdt Wilson, 94230 Cachan, France

Olivier Français

41 shared publications

ESYCOM EA2552, Université Paris-Est, Paris, France

Chen-Li Sun

27 shared publications

Department of Mechanical Engineering, National Taiwan University, Hsinchu City 300, Taiwan

Filipa Lopes

4 shared publications

LGPM, Ecole Centrale-Supelec, France

Rasta Ghasemi

3 shared publications

SATIE, Ecole normale Supérieure Paris-Saclay, 94230 Cachan, France

Publication Record
Distribution of Articles published per year 
(2016 - 2019)
Total number of journals
published in
Article 0 Reads 0 Citations Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae Pierre Bodénès, Hsiang-Yu Wang, Tsung-Hua Lee, Hung-Yu Chen,... Published: 15 February 2019
Biotechnology for Biofuels, doi: 10.1186/s13068-019-1369-z
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
This review presents a critical assessment of emerging microfluidic technologies for the application on biological productions of biofuels and other chemicals from microalgae. Comparisons of cell culture designs for the screening of microalgae strains and growth conditions are provided with three categories: mechanical traps, droplets, or microchambers. Emerging technologies for the in situ characterization of microalgae features and metabolites are also presented and evaluated. Biomass and secondary metabolite productivities obtained at microscale are compared with the values obtained at bulk scale to assess the feasibility of optimizing large-scale operations using microfluidic platforms. The recent studies in microsystems for microalgae pretreatment, fractionation and extraction of metabolites are also reviewed. Finally, comments toward future developments (high-pressure/-temperature process; solvent-resistant devices; omics analysis, including genome/epigenome, proteome, and metabolome; biofilm reactors) of microfluidic techniques for microalgae applications are provided.
Article 0 Reads 0 Citations Dielectric Characterisation of Single Microalgae Cell Using Electrorotation Measurements Yu-Sheng Lin, Sung Tsang, Rasta Ghasemi, Sakina Bensalem, Ol... Published: 07 August 2017
Proceedings, doi: 10.3390/proceedings1040543
DOI See at publisher website ABS Show/hide abstract
This study demonstrates the electrorotation of single microalgae cells captured in the center of a set of planar microelectrodes within a microfluidic device, and compares the rotational speeds of single microalgae cells obtained in experiments and theoretical calculations. Negative dielectrophoresis force and rotational electric field are generated by the microelectrode set to position and rotate single microalgae cells. The rotational speed of single microalgae cells in the microfluidic device is calculated automatically using an image-processing algorithm. The electrorotation spectra of microalgae cells enduring different periods of nitrogen starvation agree with the simulation results for low- and high-lipid content cells. These results show the great potential of using electrorotation in quantifying the dielectric characteristics of microalgae cells.
PROCEEDINGS-ARTICLE 15 Reads 0 Citations An Integrated Micro-bioreactor for enhancing the production of microalgal products Hsiang-Yu Wang Published: 17 July 2017
Proceedings of The 7th International Multidisciplinary Conference on Optofluidics 2017, doi: 10.3390/optofluidics2017-04142
DOI See at publisher website ABS Show/hide abstract
Microalgae have been studied intensively in the past decade because they have great potential in simultaneous production of biofuels and other high-value products [1]. For example, microalgae extracts have shown great antioxidant and anti-cancer effects [2] and many of the antioxidant pigments have already been commercialized. However, the production of microalgae biomass and their cellular contents strongly depends on the kind of microalgae, the cultivation condition, and the stress for inducing the accumulation of specific molecules. Conventional analyses for the cellular components of microalgae are multi-step and time-consuming, making the optimization of cultivation strategy challenging and prolonged. Therefore, a rapid and high-throughput platform for assessing the quality of microalgae culture is in great need. To rapidly investigate the effects of cultivation conditions and stresses on microalgae, micro-bioreactors have been developed and applied in enhancing the production of lipids [3] and astaxanthin [4]. The accumulation of lipids and antioxidant pigments is induced by nutrient starvation, high irradiation, high temperature, or extreme pH values. However, nutrient starvation creates a changing stress that is challenge to track and control. Oxidative stresses created by adverse environment can arrest the growth of microalgae. On the other hand, a weak electric field is reported to enhance the production of both chlorophyll and carotenoids in microalgae. Therefore, we design a micro-bioreactor integrated with microelectrodes to investigate the improvement of production of microalgal biomass and pigments by the electrical stimulus. The micro-bioreactor is composed of a glass slide containing microelectrode and multiple layers of PDMS, including the inlet layer (containing inlet microchannel), the bioreactor layer, the microelectrode layer, the outlet layer (containing outlet microchannel, and the cover layer. Two microalgae, C. vulgaris and S. abundans, are inoculated in the micro-bioreactor and fresh medium is supplied into the micro-bioreactor using a syringe pump. Twelve micro-bioreactors are operated simultaneously for the same combination of nutrient compositions and electric field to obtain statistically reliable outcomes. The biomass and total pigments are quantified by the optical density at 682 nm and 440 nm, respectively. The effect of electric field on the production of microalgal biomass is first investigated and the results are shown in Fig. 1. An electric field higher than 5 V/cm promotes the production of biomass for both microalgae. The increment of biomass is most significant in 10 V/cm and the biomass of C. vulgaris and S. abundans increases to more than 200% of the untreated culture. The combined effect of nutrient supply and electric field is also investigated. The electric field has the highest promoting effects on the biomass of C. vulgaris cultured in glucose and sucrose and S. abundans cultured in glucose...
Article 0 Reads 0 Citations Fabrication and characterization of polyelectrolyte microcarriers for microorganism cultivation through a microfluidic d... Yan-Yu Chen, Hsiang-Yu Wang Published: 01 January 2016
Biomicrofluidics, doi: 10.1063/1.4942960
DOI See at publisher website